Point spread function engineering by phase modulation is a recent approach to 3D super-resolution microscopy, with different point spread functions proposed depending on the application. It is not easy to design a phase mask from a particular desired point spread function because of missing phase information. An additional challenge is that current phase masks can either encode 3D space information or additional time information, but not both simultaneously. A robust algorithm for recovering a phase mask to generate arbitrary point spread functions is needed. We present a generalized phase mask design method and algorithm to recover phase mask designs for previously reported point spread functions. We then use the method to design a novel stretching-lobe phase mask to encode temporal and 3D spatial information simultaneously. We also present a method to fabricate prototype phase masks for proof-of-concept using multi-level light lithography and provide preliminary proof-of-concept on macromolecular dynamics.<br/>

4D super-resolution imaging of interfacial protein dynamics

2019 Fall National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.