Advanced glycation end-products (AGEs) are implicated in the pathogenesis of diabetes and age-related diseases such as Alzheimer’s disease. There is no clinically approved anti-glycation agent yet. The objectives of the study were to investigate and compare the anti-glycation effect of Psidium guajava leaf extracts (PGLETs) with that of aminoguanidine (AG) and to assess the PGLETs’ AGE-cross-link breaking ability. Bovine serum albumin (BSA) was incubated with glucose in the presence of hexane, ethyl acetate, methanol and water PGLETs at 370C for 40 days. Total immunogenic AGEs (TIAGEs), carboxymethyllysine (CML), carboxyethyllysine (CEL) and fluorescent AGEs (FAGEs) formed were measured using enzyme-linked immunosorbent assay (ELISA) and spectrophotofluorometry and the percentage anti-glycation activity of each plant extract was calculated. The ability of PGLETs to break BSA-AGE-collagen cross-links was also investigated by means of ELISA. After 40 days’ incubation, hexane, ethyl acetate and methanol PGLETs demonstrated higher anti-glycation activity against TIAGEs while hexane and the polar extracts (methanol and water) demonstrated higher anti-glycation effect than AG against CEL. Only the methanol and water PGLETs demonstrated higher anti-glycation activity than AG against CML and FAGEs. With regard to the ability of PGLETs to breakdown AGEs-protein cross-links, hexane and ethyl acetate PGLETs demonstrated higher ability to break AGE-protein cross-links than methanol and water PGLETs. Crude PGLETs have the ability to inhibit the formation of AGES and to breakdown AGE-protein cross-links. Work is underway in our laboratory to isolate bioactive compounds from PGLETs.

Anti-glycation effect and advanced glycation end-products protein cross-links breaking ability of Psidium guajava leaf extracts

2019 Fall National Meeting

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