An effective microdialysis-fluorescence biosensor system was constructed in our work. Based on the relative fluorescence intensity of vancomycin (Van) with probe, microdialysis sampling technology and flow injection analysis, a simple, time-saving, sensitive method was developed and validated for vancomycin in aqueous and plasma. The probe is based on two fluorescent groups (Danysl-) conjugated to two molecules peptides (-Lys-D-Ala-D-Ala). The strong fluorescence signals were observed from the biosensor because the probe can rapidly bind to vancomycin through hydrogen bond leading to change in electron cloud density. Under optimum conditions, the fluorescence intensity and the concentration of Van show a good linear relationship in the range 0.01–100.0 μg/mL (R<sup>2</sup> = 0.9996). The limit of detection for Van was 5.0 ng/mL (3σ). Our method was applied to analyze Van in pharmaceuticals and can be real-time monitored the concentration of vancomycin in mice. This system is expected to be a potential and practical way for the detection of concentrations of Van in clinical<br/>

Microdialysis: Fluorescence analysis system for vancomycin detection in pharmaceuticals and plasma

2019 Fall National Meeting

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