As alternatives to established carbohydrate-detection reagents (antibodies and lectins), Lectenz Bio is developing lectin-like, enzyme-derived proteins (known as Lectenz®), that target glycan sequences for which existing reagents either do not exist or are sub-optimal. Lectenz® are engineered from catalytically inactivated glycan-processing enzymes that have been optimized for high affinity towards specific glycan sequences. The conversion of such enzymes into affinity reagents is facilitated by computationally-guided directed evolution. Lectenz® are being developed for a variety of glycan detection and enrichment applications including affinity chromatography, Western blot, and immunohistochemistry. Here we demonstrate the performance and specificity of two novel sialic acid recognizing Lectenz® engineered from a sialidase enzyme.

Engineered high-specificity affinity reagents for the detection of glycan sialylation

2019 Fall National Meeting

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