Multi-photon nonlinear laser wave-mixing spectroscopy is presented as an ultrasensitive detection method for heart failure biomarkers pro-atrial natriuretic peptide (proANP) and brain natriuretic peptide (BNP). Wave mixing is an ultrasensitive optical absorption-based method, and hence, it can detect both fluorescing and non-fluorescing biomarkers. One can detect biomarkers in their native form, label-free, without the use of time-consuming labeling steps using a UV laser. The wave-mixing signal beam is strong, collimated and coherent (laser-like) and it can be collected by a simple photodetector with an excellent signal-to-noise ratio (S/N). The wave-mixing probe volume is small (picoliter), and hence, wave mixing can be conveniently interfaced to capillary electrophoresis and microfluidics separation systems to enhance chemical selectivity. The wave-mixing signal exhibits a cubic dependence on laser power and a quadratic dependence on analyte concentration, and hence, it is an ideal sensor (small changes yield big signal changes). Compared to currently available detection methods, wave mixing offers several inherent advantages such as small sample requirements, ultrasensitive detection limits, and native label-free detection of a wide range of sample including biomarkers, viruses, cancer cells, single biocells, chem/bio agents, etc. Our nonlinear multi-photon detectors can be easily configured as portable battery-powered devices that are suitable for field use.<br/><br/>

Sensitive detection of heart failure biomarkers using multiphoton laser wave-mixing spectroscopy

2019 Fall National Meeting

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