An activity-tunable biocatalyst was prepared to disrupt algal cell walls for lipid extraction. It was synthesized by co-immobilization of cellulase and lysozyme on the surface of amino-functionalized magnetic nanoparticles (MNPs) employing glutaraldehyde. The competition between cellulase and lysozyme during immobilization was caused by the limited active sites of the MNPs. The maximum recovery of activities (cellulase: 78.9% and lysozyme: 69.6%) were achieved due to synergistic effects during dual-enzyme co-immobilization. The co-immobilized enzymes showed greater thermal stability and wider pH tolerance than free enzymes under harsh conditions. They have higher catalytic efficiency for the hydrolysis of algal cell walls.  More importantly, 60% of co-immobilized enzyme activities were retained after six successive cycles.<br/>

Activity-tunable biocatalyst for algal cell wall degradation and lipids extraction

2019 Fall National Meeting

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