Access to human and other mammalian metabolites as part of agrochemical development programmes is an important component of a toxicological dossier, safety regulations for which are increasingly mimicking those for pharmaceutical testing.  Guidance from the European Food Safety Authority (EFSA) recommends testing for both the active agrochemical substances and any metabolites, which should be identified and tested separately in order to ensure the safety of crops for both human and animal consumption. Similarly, the US Environmental Protection Agency (EPA) also requires studies to define how a pesticide is absorbed, distributed, metabolized and excreted by mammals. Scalable methods to synthesize metabolites of pesticides are therefore desirable, either by chemical synthesis or through biological means.  This poster illustrates the application of a new biocatalysis kit, PolyCYPs®, to enable scalable synthesis of CYP-derived metabolites of xenobiotics. The PolyCYPs platform is comprised of a set of cloned cytochrome P450 enzymes and redox partners derived from some of the talented bacteria in Hypha’s biotransformation panel. Enzymes in the kit catalyse oxidation reactions of a wide variety of substrate types to generate multiple human and other mammalian CYP metabolites. The poster features case studies focussing on the application of PolyCYPs enzymes to produce relevant CYP-derived metabolites of drugs and agrochemicals.

Use of PolyCYPs<sup>®</sup> enzymes for accessing mammalian metabolites of agrochemicals and pharmaceutical drugs

2019 Fall National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.

Use of PolyCYPs® enzymes for accessing mammalian metabolites of agrochemicals and pharmaceutical drugs

Use of PolyCYPs^® enzymes for accessing mammalian metabolites of agrochemicals and pharmaceutical drugs