Coupling small molecules with biomolecules using diverse chemical reactions has been an increasingly salient area of research. Many reactions have been discovered, developed, and utilized to label biomolecules with organic molecules such as fluorescent dyes, biophysical probes, and drugs. Irreversible covalent labeling is an attractive property for protein labeling since it allows for unambiguous visualization of the protein after labeling.  Furthermore, conjugating small molecules with biomolecules can be useful in a greater variety of research areas, which includes drug-biomolecule conjugations. However, additional bioconjugation reactions are necessary in order to match a greater variety of applications and deal with setbacks from present methods. Here, we propose developing novel labeling techniques which would allow for site-specific, covalent labeling of proteins for a variety of potential applications—both in molecular biology and therapeutics—by using T4 Lysozyme (T4L) as a model protein. We plan to take advantage of the optimal distance between residues in protein motifs in order to perform coupling reactions on the surface of the protein.

Developing novel labeling techniques for the study of proteins

2019 Fall National Meeting

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