Bio-recognition elements are the key to affinity based bioanalytical technology. Camelid single domain antibodies (sdAb, nanobody or VHH) are increasingly popular due to their small size, high stability, ease of genetic manipulation, and ability for continuous reproducible manufacture. A new and valuable immunoreagents for the analysis of small molecular weight environmental chemicals, VHHs overcome many pitfalls encountered with conventional reagents. We have shown their use in assays for detection and quantification of pesticides, their metabolites and industrial chemicals that are comparable to polyclonal or monoclonal antibody-based assays. These antibodies can be used in classical ELISA format, and also show ease of adaption to other immunodiagnostic platforms. Several examples of nanobody-based immunoassays for chemicals will be presented, as well as their alternative applications for exposure monitoring and environmental analysis.<br/> <br/>

VHH antibodies are versatile tools for monitoring of environmental chemical contamination

2019 Fall National Meeting

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