Fatty acid esters of 2- & 3-monochloropropanediol (MCPDE) are formed during the deodorization of vegetable oils. After consumption, lipase-catalyzed hydrolysis in the gastrointestinal tract releases 2- & 3-MCPD. The International Agency for Research on Cancer (IARC) has classified 3-MCPD as possibly carcinogenic to humans. As many foods may contain MCPDE, the estimation of the external exposure is difficult. Therefore, the assessment of the internal exposure may be an alternative to estimate the external exposure more reliably. To identify possible urinary biomarkers of exposure for 2- & 3-MCPDE, twelve non-smoking participants consumed a single portion of 12 g hazelnut oil containing relatively high amounts of MCPDE (54.5 mg bound 3-MCPD/kg, 24.2 mg bound 2-MCPD/kg). During the two days before and after the controlled intake, 24-h urine samples from all participants were collected and analyzed for their levels of free 2- & 3-MCPD using GC/MS analysis with 3-MCPD-d5 as internal standard and derivatization by phenylboronic acid. In comparison to the background excretion on the days before (up to a few µg per day), the urinary excretion was largely increased on the day of controlled exposure to between 19.7 and 46.3 µg 2-MCPD and to between 13.3 and 35.0 µg 3-MCPD. The mean 3-MCPD excretion was about 3.7 % of the dose applied, whereas relative 2-MCPD excretion was about 11.3 % of the dose. The data of this study indicates the possibility to use the urinary levels of free 2- & 3-MCPD as biomarkers of exposure for dietary 2- & 3-MCPDE.

Free 2- & 3-MCPD as urinary biomarker of exposure for 2- & 3-MCPD fatty acid esters: Controlled exposure study in humans

2019 Fall National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.