Fluorescent microspheres are greatly demanded in many applications based on suspension array technology, such as immune assay, disease diagnosis, drug screening and so on. In general, these microspheres are required to be uniform in size at micrometer scale, have strong and even emission as well as surface reactive sites for interacting with analytes. In addition, different sets of microspheres with different optical codes are also indispensable in the multiplex assay. Here we report our research about the prepapration of fluorescent microspheres by introducing conjugated polymers onto the monodispersed substrate spheres. Two types of conjugated polymers were employed, poly(phenylene vinylene (PPV) and poly(arylene ethynylene) (PAE), synthesized via the Wessling method and Sonogashira coupling, respectively. The PPVs were coated onto the substrate  microspheres by adsorption of the positively charged PPV polymer precurse (pre-PPV) onto the negatively charge substrate spheres via electrostatice interaction, following by thermal elemination to convert pre-PPV into fluorescent PPV. The PAEs were introduced onto the spheres via chemical reaction. The tuning of emission color to give different optical codes and introduction of reactive sites were realized. Confocal microscopy and flow cytometry were used as the main characterizations for these spheres. The interaction and reaction between the polymers in solution and substrate spheres will also be discussed.<br/>

Preparation of fluorescent microspheres with conjugated polymers

2019 Fall National Meeting

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