The architecture of lanthanide luminescent bioprobes (LLBs) for time resolved fluorimetry immunoassay and one- (1P) or two-photon (2P) fluorescence microscopy imaging applications is currently witnessing its peak as an active field of research. Recent reports have described unprecedented in-depth high-resolution imaging. In this context, our work will present significant results in addressing the main challenges that need to be simultaneously met in a single LLB complex: 1- Development of new synthetic routes to achieve water-soluble LLBs based on pyclen and TACN macrocycles (Eu, Tb, and less common Ln such as Sm) using triazole (click chemistry) and sulfobetaine moieties; 2- Development of new functionalized macrocycles (Br, NH2, N3, alkynyl) that allow further conjugation to biomolecules in aqueous media for specific internalization in cells, or to fluorophores for FRET applications; 3- Advancement of kinetically, chemically, and photo-chemically stable LLBs in biological media with high 1P- and 2P-brigthness; 4- Excitation wavelengths that allow the use of N2 laser and Ti:sapphire laser and avoid costly quartz optics (LLBs with 336 nm and 786 nm, respectively for 1P and 2P imaging); 5- Significant 2P imaging of cells using different LLBs.

Water-soluble lanthanide (Eu, TB, Sm) bioprobes combining a pyclen or TACN framework and conjugated antennas for two-photon imaging

2019 Fall National Meeting

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