We prepared a modified carbon nitride (C3N4) with alkali-incorporation through one-pot thermal polycondensation as a new bifunctional nanozyme. The introduced chemical functionalities (i.e., KCl-bridged adjacent layers and hydroxyl group grafting) promote the two-electron reduction of dioxygen via the rapid formation of 1,4-endoperoxide species in the polymeric structure, which was in line with the theoretical simulation. The resultant metal-free photocatalyst (nanozyme) generated hydrogen peroxide (H2O2) with an apparent quantum yield of 100% (at 420 nm) and a good catalytic stability. The nanozyme of modified C3N4 exhibited the bifunctional roles that mimic: (1) GOx (glucose oxidase) that oxidizes glucose with the concurrent production of H2O2 under visible light; (2) HRP (horseradish peroxidase) that oxidizes the chromogenic substrate (TMB) to induce blue coloration in the dark. The bio-mimicking catalytic process could be facilitated within a few seconds in a continuous flow microfluidic reactor that serves as a smart and miniature device for the real-time colorimetric detection of glucose.

Bifunctional nanozyme based on modified carbon nitride photocatalyst that mimics glucose oxidase-peroxidase

2019 Fall National Meeting

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