Possessing a path to detect, image, and quantify enzymes and small molecule species linked to the disease state of human cells, is of great importance to gaining a fundamental understanding of cell behavior, as well as long-term potential to provide details of cellular function in diseased tissues. To do so requires development of enzyme- and analyte-reactive probes capable of having their fluorescence signature altered by the target in a selective and rapid fashion. We describe here the synthesis, characterization, and implementation of a family of molecular probes that respond to human oxidoreductase and glycoside hydrolase activity levels, in vitro and in vivo. The probes are either fluorescently silent and then become highly fluorescent, or their native fluorescence response at a given energy shifts to another wavelength, as a result of target-driven removal of responsive groups on a dye-based probe.  The behavior of the probes in the presence of their targets, both outside of and within human cells, including cancer cells, is presented.<br/>

Imaging of disease targets in cells via stimulus-responsive molecular probes

2019 Fall National Meeting

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