Leaf extracts of Moringa oleifera (M. oleifera) have been shown to lower blood glucose levels in both human and animal models of type 2 diabetes. Whilst there is no report on the cross-link breaking properties of these extracts, their inhibitory effect against the formation of advance glycation end products (AGEs) is also yet to be adequately investigated and confirmed. The objective of this study was to investigate the inhibitory effects of these extracts on the formation of AGEs in vitro and compare these effects with the inhibitory effect of aminoguanidine, a known inhibitor of AGEs formation and also assess their ability to break collagen-AGE-BSA cross-links. The polar extracts (methanol and water) significantly inhibited the formation of fluorescent AGEs (FAGEs) derived from fructose after incubation for both 20 and 40 days. In the case of total immunogenic AGEs (TIAGEs), at 20 days the highest inhibitory effect was observed in the hexane extract which was significantly higher than aminoguanidine whereas after 40 days’ incubation the highest inhibitory effect was obtained in the methanol extract and this was also significantly higher than that of aminoguanidine. All other extracts and aminoguanidine demonstrated similar inhibitory effect against TIAGEs as aminoguanidine after incubation for 40 days. After both 20 and 40 days’ incubation, the polar extracts had comparable inhibitory effect as aminoguanidine against the formation of BSA-fructose derived carboxymethyllysine (CML). For cross-link breaking activity, all M. oleifera leaf extracts demonstrated the ability to break BSA-fructose derived collagen-AGE-BSA cross-links.

Cross-link breaking activity and inhibitory effect of Moringa oleifera leaf crude extracts on fructose-derived advanced glycation end products

2019 Fall National Meeting

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