An approach, based on LCMS/MS, for simultaneous determination of fungicide has been developed and validated in various matrices, i.e., distilled water, receptor fluid, methanol, and 1.5 M KOH in 20% ethanol. In this research study we aimed to establish a baseline sensitivity data which was investigated for dermal in-vitro absorption and its toxicological application. Dermal absorption is an integral part of non-dietary human safety risk assessments for agrochemicals. In-vitro dermal absorption studies offer a valid alternative for in-vivo studies and are conducted with skin from different species such as human, rat, and pig. The analytical method allows acceptable and satisfactory results with good linearity, trueness, precision, and negligible matrix effect. Results demonstrate complete traceability throughout the procedure. Calibration curve for all matrices were linear within a range of 0.00014 mg/L and 0.07133 mg/L with a correlation coefficient (R2) of &gt;0.999. The limit of detection (LOD) and the Limit of Quantification (LOQ) were 0.000025 mg/L and 0.00006 mg/L respectively. Mean relative standard deviation (%RSD) was &lt;15.0 %, in terms of precision and accuracy in distilled water, receptor fluid, methanol, and 1.5 M KOH in 20% ethanol. The mean accuracy (% recovery) was within a range of 91.67% and 93.86%, in distilled water, receptor fluid, methanol, and 1.5 M KOH in 20% ethanol. The validated method was successfully applied to the real samples of dermal absorption in-vitro study, for the analysis of fungicide (fenpropidin) and was found that the method has a promising application in the analysis of samples of in-vitro toxicological studies.

Establishing baseline sensitivity data using LCMS/MS to investigate dermal in-vitro absorption toxicological application

2019 Fall National Meeting

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