Nucleic acids experience a variety of perturbations, which include strand cleavage and ligation, local conformational changes, base damage, modification and flipping, as well as structural and environmental perturbations that are induced upon protein and low MW ligand binding. Additionally, nucleosides and nucleotides are involved in numerous biochemical transformations and regulatory processes. Isomorphic responsive fluorescent nucleoside analogs, which can serve as faithful surrogates, are powerful probes for investigating nucleic acids structure, dynamics, recognition and damage as well as metabolic processes. Such modified nucleosides can also serve as mechanistic probes, shedding light on biological pathways and providing guiding design principles for potential therapeutics. The lecture will present the design and synthesis, as well as the structural and photophysical properties of new fluorescent isomorphic nucleoside analogs as well as their utilization for the fabrication of "real-time" fluorescence-based discovery and biophysical assays.

Isomorphic and isofunctional fluorescent nucleosides

2019 Fall National Meeting

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