We report a facile, high-resolution aerosol-based electrophoretic approach to quantitatively study (1) hyaluronic acid (HA) and study its crosslinking reaction, and (2) metal-organic framework (MOF) as molecular drug carrier. Electrospray-differential mobility analysis (ES-DMA) was employed, where mobility size distributions, number concentrations, molecular mass distributions and polydispersity index of HA and MOF were obtained successfully by ES-DMA. Orthogonally, size exclusion chromatography (SEC) was employed for data comparison on a semi-quantitative basis. Using ES-DMA, the 1,4-butanediol diglycidyl ether (BDDE)-induced crosslinking of HA was also able to be successfully characterized through a time-dependent study. Electrospray-differential mobility analysis coupled to aerosol particle mass analyzer (ES-DMA/APM) was employed to successfully develop metal-organic framework (MOF)-based targeted drug delivery platform. A direct quantification of the loading of isoprofen (the representative molecular drug) on individual MOF particles achieved using the ES-DMA/APM. The interaction with phosphate ions in the medium and the corresponding drug release was successfully identified through a time-resolved study. The corresponding methodology provides a proof of concept for the study of formulation strategies to drug delivery systems.

Quantifying hyaluronic acid and metal-organic framework for biomedical applications using gas-phase electrophoresis

2019 Fall National Meeting

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