Epithelial cell adhesion molecule (EpCAM) is considered as the biomarker of circulating tumor cells that play an important role in tumor metastasis. Detection of EpCAM is vital for personalized diagnosis and therapy, but is challenging because of its extremely low concentration in peripheral blood. In our work, an electrochemical aptasensor has been developed to quantitatively detect EpCAM at low concentration. Mesoporous silica nanoparticles were synthesized and modified with CdSe quantum dots and aptamer specific to EpCAM as a signal reporter. EpCAM was captured by a gold electrode modified with another aptamer specific to EpCAM, and then further recognized by the signal reporter. By means of stripping square wave voltammetry, 10 aM EpCAM can be successfully determined and quantified based on the newly developed aptasensor. The detection linear range is from 10 aM to100 pM, which has the potential to meet the requirements for different applications. The principle can also be extended to other biomarkers when suitable aptamer or antibody is available. The aptasensor can be a potential tool for diagnosis, therapeutic evaluation and personalized medicine for cancer patients.<br/>

Sensitive electrochemical aptasensor for EpCAM by mesoporous silica nanoparticles and quantum dots signal amplification

2019 Fall National Meeting

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