The characteristic constituent and anti-inflammatory activity of 5-<i>n</i>-alkylresorcinols (ARs) from 21 wheat bran samples in China were investigated in this study. The amount of ARs ranged from 697 μg/g to 1732 μg/g in the tested samples, which were composed of five different homologues. Among these homologues, C19:0 and C21:0 were the most abundant, followed by C17:0, C23:0 and C25:0. Moreover, the mRNA expression of IL-1β, IL-6 and TNF-α in LPS-activated RAW264.7 macrophage cells were significantly inhibited by ARs supplementation. The molecular mechanisms behind its anti-inflammatory activity could result from the suppression of nuclear factor-κB (NF-κB) and JNK/MAPK activation. ARs treatment notably decreased NF-κB p65 nuclear translocation and inhibitor κB (IκBα) kinase and JNK phosphorylation. Additionally, ARs homologues C17:0 had been proven to be the main active constituent. The results from this study could be used to promote the comprehensive utilization of wheat and its by-products in improving human health.<br/>

Isolation and purification of 5-n-alkylresorcinols from 21 different wheat varieties and its inflammation inhibitory potential under LPS induced RAW264.7 macrophages

2019 Fall National Meeting

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