Supported lipid bilayers are valuable tools for investigating membrane components. Several supported lipid bilayer techniques allow work with membrane components, such as membrane proteins, which is difficult to accomplish using vesicles. For example, several separation methods have been developed in supported lipid bilayers. Although supported lipid bilayers provide many techniques, the solid support may be problematic when measuring transport or transmembrane signaling. We have developed a method to convert supported lipid bilayers into vesicles using fast buffer flow. Using a microfluidic device, we stripped a fluorescently labelled supported lipid bilayer from its glass support. This stripping was observed directly on the glass slide using fluorescence microscopy, and the stripped lipids were fluorescently detected in solution.

Supported lipid bilayer stripping by buffer flow

2019 Fall National Meeting

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