Sialylation to glycoconjugates plays as biologically important switches in nature. For matrix-assisted laser desorption/ionization–mass spectrometry (MALDI-MS) analysis of sialylated glycans and glycoconjugates demands additional modification-purification processes of the carboxylate group of sialic acid residue to avoid in-source or post-source decay. To overcome this problem, I find a versatile and high-sensitivity matrix system, aniline derivative/2,5-dihydroxybenzoic acid/alkali metal salt, for MALDI-MS analysis of sialyloligosaccharides and sialylglycoconjugates without any modifications. This new matrix system affords a simple adduct ion signal from the femtomole range of the analytes tested by MALDI-MS in reflectron mode. Besides, the laser power-dependent in-source decay and post-source decay pattern of the sialylated analytes with this matrix system allows a pseudo MS<sup>3</sup> strategy for in-depth sequencing studies of the analytes.

Novel matrix system for reflectron mode MALDI-TOF and TOF/TOF MS analysis of unmodified sialylated oligosaccharides and glycopeptides

2019 Fall National Meeting

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