Brain is vulnerable to lipid peroxidation during oxidative stress. Malondialdehyde (MDA), as a biomarker of lipid peroxidation, is prone to cross-link with biomacromolecules to destroy their functions, subsequently causing brain diseases. However, precise determining brain MDA in vivo remains a challenge due to the complex construction of the brain and lack of desirable techniques. Thus, we present a novel polymer carbon dots material (BH-CDs) with benzoyl-hydrazide as precursor. This functional nanoparticle BH-CDs attaching acyl-hydrazine on the surface directly formed in one-pot through dehydration, which could identify MDA via a photo-induced electron transfer (PET) mechanism. It afforded attractive traits of a simple preparation, large two-photon absorption cross-section, exceptional biocompatibility, as well as the ability of traversing blood brain barrier. Notably, BH-CDs could specifically distinguish MDA from other competing biological species, especially reactive carbonyl species. Therefore, we relied on BH-CDs to unravel noticeably increased MDA in PC12 cells under stimulation with H2O2. Remarkably, benefitting from the favorable imaging performance of BH-CDs, we unprecedentedly real-time portrayed MDA in mice brains with cerebral ischemia-reperfusion injury (CIRI), and witnessed a positive correlation between MDA and CIRI. Overall, we provide a meritorious nanolight serviceable to reveal the pathogenesis of MDA-related brain diseases.

Specific, in vivo two-photon fluorescence imaging of malondialdehyde in mice brains using an easily-prepared nanolight

2019 Fall National Meeting

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