This presentation details the use of gold nanostars (GNSs) in lateral flow assay (LFA) applications and the influence of the morphology of the stars on the sensitivity and detection limit of the LFA toward human chorionic gonadotropin (hCG) hormone.  The gold nanostars used in this study were synthesized through a seed-growth method and functionalized with a self-assembled monolayer (SAM) generated from a custom-designed bidentate carboxylate-terminated dithiol, 16-(3,5-bis(mercaptomethyl)phenoxy)hexadecaonic acid (BMPHA).  Functionalization of the surface of the stars with the SAM enables bioconjugation (e.g., with antibodies) and utilization in various biological applications, such as nanosensors, biological assays, and drug delivery.  The functionalized GNSs were characterized with UV-Vis spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectroscopy (EDS), and Fourier-transform infrared (FTIR) spectroscopy.  The engineering and evaluation of hCG-targeted bio-sensing nano-platforms using these colloidally stable GNSs will be described.

Impact of gold nanostar morphology on bioassay sensitivity

2019 Fall National Meeting

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