Bioluminescence imaging with luciferase-luciferin pairs is commonly used for monitoring biological processes <i>in vivo</i>. While popular, this technology has been historically limited to visualizing only a few cell types or features at a time. Few distinguishable, tissue-penetrant probes exist, precluding efforts to visualize immune function and other multicellular processes in deep tissue. To address this void, we developed new classes of luciferins comprising diverse steric and electronic modifications. We further engineered luciferases to selectively process the analogs. The resulting enzyme-substrate combinations can be readily differentiated from existing bioluminescent probes based on unique patterns of light emission. Examples of the engineered luciferins and luciferases, along with their application <i>in vivo</i>, will be discussed.

Multi-component bioluminescence imaging with diverse luciferin architectures

2019 Fall National Meeting

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