Tetracycline and analogues are among the most used antibiotics in the dairy industry leading to their presence in commercial milk. The purpose of this study is to develop a quick extraction method for the tetracycline analogues from milk with triofluoroacetic acid and the results are compared with the SPE extraction. Tetracycline analogues from the extracted samples were separated and detected by an HPLC-UV system with maximum absorbance wavelength = 355 nm. Calibration curves for tetracycline analogues ( MC, TC, DEM, CTC and DC) were prepared from standard solutions with concentration range from 0.0320 mg/mL to 2.034 mg/mL Good linearity was shown for each analogue ( r^2 value range from 0.9905-0.9967). Percent recovery for MC, TC, DEM, CTC and DC was: 80.67%, 48.82%, ~90%, 78.54% and 86.3% respectively. The results are comparable with the SPE extraction except for TC due to the interference from the milk matrix.

Development of a simple extraction method for tetracycline analogues from milk with UV detection

2019 Fall National Meeting

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