Mashua (Tropaeolum tuberosum R. & P.) is an Andean tuber from Peru with a high economic value as a food and crop with medicinal properties, which grows from 1,500 to 4,200 (m.a.s.l.) and has a great diversity in morphology and color, which varies from cream to dark purple. Within the genotypes of Mashua, the purple-colored Mashua, which contains a high content of phytochemicals such as anthocyanins and glucosinolates, would have antioxidant, anti-inflammatory and anticancer properties. The objective of the study was to evaluate the in vitro cytotoxic and antioxidant activity of a purple-colored Mashua extract against the prostate (DU-145) and human breast (MCF-7) cancer cell lines. A purple-colored (peel/flesh, purple/purple) genotype of Mashua (Tt23) from the ILLPA Puno Agricultural Experiment Station, Peru, located at 3,815 (m.a.s.l.) was evaluated. From a lyophilized sample, extraction was performed with Methanol/acetone/water (45/45/10, v/v/v). The content of total phenolic compounds, flavonoids and antioxidant capacity was determined by the Folin-Ciocalteu method, colorimetric method of aluminum chloride and DPPH assay, respectively. Cytotoxic activity was evaluated using the lactate dehydrogenase (LDH) release assay after 12 and 24 hours of exposure with different concentrations of Mashua extract (100, 250 and 500 µg/mL). Statistical analysis of the experimental data was carried out using one-way ANOVA followed by a Tukey test (P&lt;0.05). According to our results, the Mashua extract showed a content of polyphenols, flavonoids and antioxidant capacity of 19.531±0.210 mg AGE/g, 2450±84.507 µg CE/g and 1107.614±74.013 nmol TEAC/g. A greater cytotoxic activity was observed at 24 compared to 48 hours of exposure with different concentrations of Mashua extract (100, 200 and 500 µg/mL) in DU-145 cells (P&lt;0.05, Table 1). After 24 hours of exposure, the Mashua extract (100 µg/mL) showed a significant increase in cytotoxic activity in DU-145 compared to MCF-7 cancer cell line (P&lt;0.05, Table 1). Our results suggest that the Andean Mashua extract has an increased cytotoxic activity against prostate (DU-145) cancer cell line, mediated by its high flavonoid content and antioxidant capacity.

Cytotoxic and antioxidant activity from Andean mashua (Tropaeolum tuberosum R. & P.) extract against prostate (DU-145) and human breast (MCF-7) cancer cell lines

2019 Fall National Meeting

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