As stereoisomers of a compound can have different bioactivity, toxicology and environmental fate behavior, analysis of stereoisomers of pesticides and their degradates/metabolites has gained growing attention in environmental fate and metabolism studies during the regulatory registration process for thorough consumer and environmental safety assessment. However, chromatographic separation and quantitation of stereoisomers, especially enantiomers, can be very challenging due to their structural similarity. Recently, supercritical fluid chromatography (SFC) has shown to be a powerful tool for separation of chiral molecules. When coupled with mass spectrometry (MS) as the detector, the high sensitivity of MS allows for analysis of trace-level residues in environmental fate and metabolism samples and selective monitoring of the ions of interest eliminates the intensive efforts needed to isolate the stereoisomers from complex degradates/metabolites mixture. Here, we present case examples where an analytical strategy based on SFC-MS was used to analyze the stereoisomers of pesticides and their major degradates/metabolites in environmental fate and metabolism studies, and how these data could support regulatory assessment.

SFC-MS based analytical strategy for stereoisomer analysis in environmental fate and metabolism studies

2019 Fall National Meeting

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