“Fudegaki (Fudegaki persimmon)” is a type of incomplete sweet persimmon. It has a higher sugar content than other persimmons. The leaves and fruits of other persimmons have been well studied, whereas those of Fudegaki have not. We investigated the antioxidant activities, polyphenol contents, and ascorbic acid contents of leaves and fruits of Fudegaki. Leaves were harvested from Kota-cho, Nukata-gun, Aichi prefecture (where this persimmon predominates) at the beginning of May. The harvested leaves were steamed and dried, and 100 mg samples were extracted with 25 mL of distilled hot water at 100°C for 10 minutes. The extract was filtered through filter paper. Near-infrared spectroscopy examination was performed to categorize the Fudegaki fruits into sweet, half-astringent, and astringent groups. The fruits were extracted with 80% ethanol or acetone. Antioxidant activities were examined by α, α-diphenyl-β-picrylhydrazyl (DPPH) radical scavenging assay, total polyphenol contents were determined using the Folin–Ciocalteu method, and ascorbic acid was measured using the hydrazine colorimetric method. Leaf extracts showed 52.6% DPPH free radical scavenging activity. Total polyphenol compounds and ascorbic acid content was 67.1 μg/mL equivalent to catechin content and 4.15 μg/mL, respectively. Total polyphenol content of fruits in the sweet, half-astringent, and astringent groups extracted with 80% ethanol was 71.3 mg, 101.5 mg, and 333.3 mg equivalent to gallic acid/100 mg fresh fruit, respectively. When acetone was used for the extraction of fruit, the total polyphenol equivalent to gallic acid/100 mg fresh fruit was 5.4 mg, 5.1 mg, and 3.4 mg for the of sweet group, half-astringent group, and astringent group, respectively. Total polyphenol content of the ethanol extracts was higher than that of acetone extracts in all groups. DPPH radical scavenging activity was 589.8, 1195,3, and 4070.1 μmol equivalent to Trolox/100 mg fresh fruit in the sweet group, half-astringent group, and astringent group, respectively. The results demonstrate that the leaves and fruits of Fudegaki include polyphenols and compounds with DPPH radical scavenging activity.<br/>

Antioxidant activities and constituents of leaves and fruits of a Japanese persimmon (Fudegaki)

2019 Fall National Meeting

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