Ganoderma formosanum, a traditional medicinal mushroom, has been used in Asian for more than 2000 years. Extraction of Ganoderma formosanum possesses diverse bioactivities, such as anti-inflammatory, antitumor, immunomodulatory and skin whitening functions. Skin is the biggest organ in a human body, also it is the first and most important defense barrier to the external contaminants. Multitudes of chemicals can be absorbed into skin, so long-term exposure to the ambient pollutants will result in toxicity. Particulate matter (PM) is a common index of air quality, which can be divided into fine particulate matter (PM2.5) and coarse particulate matter (PM10), according to its aerodynamic diameter. Epidemiological studies have proven that fine particulate matter (PM2.5) exerts negative effects and is unable to be blocked by our defensive system. Previous studies indicated that PM brings about the generation of intracellular reactive oxygen species (ROS). ROS, including superoxide and hydroxyl radical, is a natural byproduct of cell oxidative phosphorylation, and a persistent imbalance between ROS generation and elimination can result in tissue impairment. The study discusses the potential of protection against PM2.5-induced ROS generation and cell viability with extraction of Ganoderma formosanum submerged fermentation mycelium in vitro human keratinocyte (HaCaT cell), meanwhile assesses the toxicity of PM2.5 to animals by using zebrafish in vivo model. Our results show that PM2.5 treatment promotes the intracellular ROS level and decrease cell viability both in a dose-dependent manner. The same effect can also be observed in zebrafish model, and PM2.5 will damage its cardio-development, which causes the reduce of heart-beating rate. Pretreatment of Ganoderma formosanum extraction ameliorates intracellular ROS generation and apoptosis. Taken together, these findings imply that antioxidant activities of Ganoderma formosanum extraction may play an important role in the protection of PM-caused cell damage.

Potential evaluation of Ganoderma formosanum extract against PM2.5-induced ROS generation

2019 Fall National Meeting

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