Disordered proteins are widely present in proteomes, and their complex dynamics and non-covalent/covalent chemical reactions are critical to proper cell function. Here, fluorescence approaches for studies of these complicated systems and their reactions will be discussed. A particular focus is on single-molecule FRET which can provide nanoscale information about dynamics during interactions and folding. Insights into complex binding-folding landscapes and additional complexity resulting from multicomponent interactions and 2D molecular crowding will be discussed. In addition, ensemble and single-molecule studies of protein liquid-liquid phase separation, and resulting reentrant phase transitions and non-equilibrium substructure formation will be discussed. These protein droplet formation/dissolution reactions are relevant to dynamic compartmentalization and function in cells. Overall, the presentation will highlight the strengths of such fluorescence approaches to map dynamic nanoscale to mesoscale complexity related to protein disorder and corresponding function.

Fluorescence studies of protein disorder, single molecules to droplets

2019 Fall National Meeting

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