Malachite green (MG) is a triphenyl methane cationic dye used extensively in aquaculture as a parasiticide/fungicide against protozoan and fungal infections in farmed fish. The use of this dye generates a lot of concern because of its known toxic effects, including organ damage, mutation and developmental abnormalities in mammals. In this study, three degrading bacterial strains M6, M10 and M12 with MG-degrading ability were isolated, identified and characterized from the sediment of milkfish (Chanos chanos) culture ponds. The degradation of MG and the changes in the bacterial community in milkfish culture pond sediment were revealed. Phylogenetic analysis using 16S rRNA gene sequences suggested that bacterial strain M6, M10, M12 is closely related to Zhouia amylolytica, Tenacibaculum mesophilum, and Enterobacter cloacae, respectively. The addition of bacterial strains M10 and M12 can enhance the MG degradation in milkfish pond sediment. Continuous addition experiments revealed that bacterial strain M10 was more persistent and may provide more contribution for MG degradation. Results of this study suggest that bacterial strains M10 and M12 provides a potential solution for treatment of sediment of high salinity aquaculture ponds with MG contamination.

Microbial degradation of malachite green in milkfish pond sediments

2019 Fall National Meeting

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