Ginkgo biloba leaf extract possesses many active ingredients whereas with ginkgolic acid, which leads to serious allergies and toxicity, could contribute to an excessive expansion of capillaries. The purpose of this study aims to immobilize laccase on the nylon carrier using electrospinning and to degrade the ginkgolic acid within G. biloba leaf extract by enzyme hydrolysis. An enzyme immobilization system for degradation of ginkgolic acid in G. biloba leaf extract was established. Laccase which was immobilized onto the nylon 6,6 nanofiber membrane with multi-walled carbon nanotubes (LNFM-MWCNTs) exhibited a significant efficiency for the catalyzation of ABTS then nylon 6,6 pellet. The optimum temperature and pH for ABTS catalysis was 40oC and 5, results revealed that the endurances of LNFM-MWCNTs to pH and temperature were improved. Operational reusability and storage stability was confirmed for more than 10 reactions and 40 days. The kinetic parameters, including rate constant (K), the time (τ50), the time (τcomplete), Km and Vmax were determined, which were 0.07 ± 0.01 min−1, 8.97 ± 0.55 min, 45.45 ± 2.79 min, 0.51 ± 0.09 mM and 0.05 ± 0.00 mM/min*mg, respectively. This study demonstrates the potential of laccase immobilized system in enhance the enzyme activity and the endurances about environmental factors, to remove ginkgo acid in G. biloba leaf extract.

Immobilized of laccase for ginkgolic acid degradation

2019 Fall National Meeting

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