To clarify anti-inflammatory effect of Okinawan vegetable methanol extracts, we examined the nitric oxide production and some kinds of pre-inflammatory markers such as IL-6 and TNF-α from mouse macrophage cells (RAW264) by the addition of Nishi-yomogi collected at Isigaki and Kume Island and Hosoba-wadan extracts. In addition, we studied the anti-inflammatory impact of extracts using carrageenan-induced footpad edema animal model. In the case of in vitro study, NO and cytokine concentrations of supernatant were assayed with Griess method and ELISA kit, respectively. Gene expressions of iNOS, COX-2, and NF-κB were estimated by quantitative PCR. In the study of footpad edema animal model, 6 week-old male SD rats were treated with intraperitoneal administration of extracts, and induced edema by injection of carrageenan on right footpad. Footpad volume before and after administration of carrageenan was measured. Data are presented as means±SEM. The statistical significance of difference was evaluated by Tukey-Kramer HSD test. Differences at P &lt;0.05 were considered to be significant. NO and TNF-α productions of the Kume Nishi-yomogi group were significantly lower (P &lt;0.05) than those of other groups, and also IL-6 production of the Kume Nishi-yomogi group was significantly lower (P &lt;0.05) than that of control. The iNOS and COX-2 gene expressions of Kume Nishi-yomogi group were significantly downregulated (P &lt;0.05) compared to those of other groups. While, the footpad edema of rats administered extract showed no significant difference between groups, but the tendency of decreased edema was observed in Kume Nishi-yomogi group. From these results, it is expected that Nishi-yomogi from Kume Island might be effective in the alleviating inflammation.

In vitro and in vivo studies on anti-inflammatory effects of traditional Okinawan vegetable methanol extracts

2019 Fall National Meeting

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