In this presentation, we will introduce a new carbon-based peroxidase mimic, N and B co-doped reduced graphene oxide (NB-rGO), which show unprecedentedly high peroxidase-like activity without oxidase-like activity, and that has up to 1000 times higher catalytic efficiency than does undoped rGO. The high catalytic activity of NB-rGO is explained by density functional theory (DFT) by calculating Gibbs free energy change during the peroxide-decomposition reaction. Acetylcholine and C-reactive protein are successfully quantified with high sensitivity and selectivity, which were comparable to or better than those obtained using natural peroxidase. Furthermore, NB-rGO which doesn’t have oxidase-like activity is proved to have higher sensitivity toward acetylcholine than Pt nanoparticles having oxidase-like activity. This work will facilitate studies on development, theoretical analysis for rational design, and bioassay applications of enzyme mimics based on nanomaterials.

Strong candidate graphene based materials to replace natural peroxidase in sensitive and selective bioassays

2019 Fall National Meeting

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