The phospholipase A2 (PLA2) is a large group of biologically active enzymes that can hydrolyze lipid membranes. PLA2 is overexpressed in some disease conditions including colon, breast, lung, pancreatic and prostate cancer. It is thus important to study the PLA2 interaction with lipid membranes in order to develop new sensitive diagnostic tools and drug delivery methods for diseases like cancer. Currently, liposomes are utilized to study the enzyme-membrane interaction. However, liposomes lack long-term stability, defined shape and size, and robustness. One attractive alternative for the liposomal assay is to use lipid membranes supported on spherical solid microspheres. In this work, we demonstrate the use of lipid-coated microspheres to study the PLA2-lipid membrane interaction. In our approach, we utilize a sensor dye encapsulated silica microparticles and the enzyme-membrane interaction is detected by flow cytometry. In the presence of PLA2, lipid membranes are hydrolyzed thus released the encapsulated dye. The extent of dye release is measured via flow cytometry and it is used to investigate the degree of enzyme-membrane interaction.

Supported lipid bilayer-based biosensor to detect phospholipase A2 -lipid membrane interaction

2019 Fall National Meeting

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