Nanomaterials possessing peroxidase-like activity have received great interest due to their potential to replace natural peroxidase in biotechnology. While a variety of nanomaterial-based peroxidase mimics have been developed, their relatively low catalytic efficiency is still an issue to be overcome to realize their practical utilization. Herein, we describe a new carbon-based peroxidase mimic, N and B co-doped reduced graphene oxide (NB-rGO), which show unprecedentedly high peroxidase-like activity without oxidase-like activity, and that has up to 1000 times higher catalytic efficiency (kcat/Km) than does undoped rGO. Density functional theory (DFT) analysis explained the high catalytic activity of NB-rGO theoretically by calculating Gibbs free energy change during the peroxide-decomposition reaction. NB-rGO was then used to construct bioassay systems to detect acetylcholine (ACh+) by oxidase-coupled biosensing, and C-reactive protein (CRP) by immunoassay. NB-rGO successfully quantified both target molecules with high sensitivity and selectivity, which were comparable to or better than those obtained using natural peroxidase. This work will facilitate studies on development, theoretical analysis for rational design, and bioassay applications of enzyme mimics based on nanomaterials.

N and B co-doped graphene: Suitable candidate to substitute natural peroxidase in sensitive and selective bioassays

2019 Fall National Meeting

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