Advancement of therapeutic protein therapy has been hindered by the poor stability and short half-life of proteins in vivo. There is emerging evidence that biocompatible zwitterionic materials can prevent non-specific interactions within proteins systems. Here, a form of zwitterionic hydrogel is synthesized from poly sulfo-betaine methyl acrylate (SBMA) using an inverse emulsion, radical polymerization reaction. The zwitterionic microgel reaction was characterized using1H NMR. Microgel particles were loaded with a model protein, serum albumin, and the protein loading efficiency was studied. Furthermore, the release rates of the protein from the synthesized microgels were studied at different monomer to crosslinker ratios. The microgel particles were analyzed for their size, regenerative characteristics, protein stability, and delivery properties. Furthermore, in vitro studies were carried out to evaluate toxicity and immunogenicity of the microgels.

Synthesis, characterization, and evaluation of a zwitterionic microgel for therapeutic protein delivery

2019 Fall National Meeting

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