Glucose is the prime precursor for lactose synthesis, and the availability of glucose in bovine mammary gland plays a crucial role in milk production of dairy cows. In the present study, the effects of insulin on glucose uptake and the underlying regulatory mechanisms were investigated in MAC-T [a bovine mammary epithelial cell (BMECs) line] cells. MAC-T cells were treated with different levels of insulin (0, 250, 500, 750, 1000 ng/mL) for varying time points (2, 12, 24 and 48 h) after a 24 h serum starvation. Compared with the control group (0 ng/mL insulin), cell viability was overall enhanced by the addition of insulin, with the highest viability at 500 ng/mL insulin at most of time points. Addition of 500 ng/mL insulin significantly increased glucose uptake at all time points (P &lt; 0.05). Also, the mRNA and protein abundance of GLUT8 were both significantly enhanced (P &lt; 0.05) after 12 h of insulin treatment and reached the highest level at 24 h. Furthermore, insulin remarkably stimulated the translocation of GLUT8 from cytoplasm to the plasma membrane. In summary, the results suggested that insulin-induced glucose uptake in BMECs was likely mediated through enhancing the expression and translocation of GLUT8.

Insulin regulates the expression and translocation of GLUT8 to increase glucose uptake in bovine mammary epithelial cells

2019 Fall National Meeting

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