Abasic (Ap) sites are one of the most common lesions in DNA. In biological systems, Ap sites can undergo either spontaneous or amine-catalyzed strand cleavage to generate an electrophilic alpha, beta unsaturated aldehyde (alkenal) at the 3’-terminus of the strand break. We will present evidence that spermine-mediated strand cleavage at an Ap site in duplex DNA can result in the formation an interstrand cross-link adjacent to the strand break <i>via </i>reaction of the 3'-alkenal unit with aguanine residue on the opposing strand. This chemistry may be biologically significant because interstrand cross-links can be cytotoxic, mutagenic, or induce genomic instability.

Interstrand cross-links at strand breaks derived from abasic sites in duplex DNA

2019 Fall National Meeting

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