Microphysiological systems replicate human organ function and are promising technologies to transform both pharmacoengineering and regenerative medicine. Because microphysiological systems require complex anatomical structures and heterogeneous cell populations, a major challenge remains to manufacture and operate these products with reproducible and standardized function. Monitoring of microphysiological systems requires non-destructive, continuous biosensors and imaging techniques. Herein, we will present three different methodologies for monitoring blood-tissue interfaces in different microphysiological systems. First, a microphysiological model of human breast epithelium was engineered and photonic oxygen sensors were implanted in the engineered tissue. Photonic oxygen sensors were implanted in each 3D tissue model to monitor oxygen levels in real-time by fluorescence lifetime spectroscopy. Next, a simplified model of the human blood-brain barrier is engineered and integrated with micorelectrodes to monitor both transendothelial resistance and generate impedance tomographs of the barrier under development and dysfunction. Lastly, we will present a completely non-invasive method to interrogate microvascular development in 3D tissue constructs <i>via </i>perfusion pressure recording and modeling. By recording the perfusion pressure through an expanding microvasculature, we can correlate the pressure change to total vascular volume and interconnectivity; <i>i.e. </i>a proxy measrument of tissue development. Ultimately, with such tools, the extent tissue development and function, can be autonomously determined and optimized by correlating physical and chemical sensor outputs with markers of physiological performance

Multi-modal sensing for measuring tissue development and function in microphysiological systems

2019 Fall National Meeting

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