The consumption of vegetables plays an important role in the daily nutrition. A large part is represented by the order of <i>Brassicales</i> such as cabbage, horseradish, or water cress. These species contain a high amount of glucosinolates (GSL) which are secondary sulfur-containing plant metabolites. These β-thioglucoside-N-hydroxysulfates protect the plants from predators and contribute to aroma and taste as well. An important issue related to these compounds is their degradation mechanism(s) during food processing. Previous studies showed that a heat-treatment of GSL in different matrices led to pleasant desired aroma impressions. However, also undesired toxicologically relevant compounds, such as nitriles and epithionitriles are formed. The aim of the present study was to consider risks and benefits using GSL as a source of natural aroma compositions, with a special focus on sinigrin (2-propenyl GSL) and glucotropaeolin (benzyl GSL).<br/>After heat-processing of the GSL in phosphate buffer at pH values of 5.3, 7, or 9, the volatile compounds were extracted via solvent assisted flavor evaporation (SAFE) technique. Screening for aroma-active compounds was performed by aroma extract dilution analysis (AEDA) based on high resolution gas chromatography-olfactometry (HRGC-O). Afterwards, the odor-active areas with the highest flavor dilution (FD) factors were identified by comparison of their retention indices on two columns of different polarities, their odor quality and intensity at the sniffing port as well as their mass spectra (EI and CI mode) with data of reference compounds in an in-house database containing &gt; 1000 aroma-active volatiles. Finally, these compounds were quantitated by means of comprehensive two-dimensional GCxGC-ToF-MS on the basis of stable isotopically labeled standards.<br/>In summary, the lecture will present glucosinolates as a possible source for natural flavor compositions in the future, which could fulfill the demand by consumers to have all natural food products.

Thermally induced generation of desirable aroma-active and undesirable toxicologically relevant compounds from glucosinolates

2019 Fall National Meeting

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.