Improved cryopreservatives are necessary to enable complete recovery of living cells and tissue after frozen storage. A key challenge in cryopreservation is reducing osmotic and mechanical toxicity on cells during freezing while minimizing the toxicity of cryoprotectants. We synthesized a new class of polyether-based materials with statistical control of polymer structure and charge. Excellent biocompatibility was demonstrated, and cryoprotective efficacy was evaluated for each material using model mammalian cell lines. Optimization of composition led to significant advances in cryoprotective performance and an emerging understanding of some aspects of the complex mechanism of cryopreservation with polymeric cryoprotectants.

Polyether-based cryoprotectants for quantitative post-thaw recovery of mammalian cells

2019 Fall National Meeting

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