Synthetic environmental toxins are widely distributed with adverse effect on human health. Persistent organic pollutants(POPs) are synthetic chemicals that are resistant to degradation through chemical, biological and photolytic process. Meanwhile, several animal models like mouse or rats are used to study the effect of toxic materials. Zebrafish, well-known vertebrate organism, has strength as novel animal model in research of disease or toxicology due to its rapid ontogeny process and easier genetic and proteinic manipulation than other animal models. Also, it is beneficial to examine several exposure-effect from its water-survival nature. Here, 5 kinds of persistent organic pollutants mixture (<i>β</i>-HCH, Heptachlor, Hexachlorobenzene, 4, 4’-DDT and Chlordane) are directly treated to isolated mitochondria of zebrafish’s liver to analyze its effect on mitochondrial activity. After adding POPs mixture, activity of each mitochondrial complex I, II, III and IV was measured immediately to examine the short-term exposing effect by spectrophotometric method. As a result, higher concentration of POPs treated mitochondria exhibited much more enhanced enzymatic activity than non-treated one except complex III. In the case of complex III, conversion of hexachlorobenzene contained in POPs into redox-active form of tetrachloro-1,4-benzoquinone in oxidative condition is competitively able to inhibit the Q binding site of mitochondrial complex III resulting in decreased enzymatic activity. Furthermore, competitive inhibitory action was confirmed by steady-state kinetic analysis of complex III with different concentrations of substrate and POPs mixture. Accordingly, production of ATP was decreased in POPs treated mitochondria compare to non-treated one. This study suggests that brief in-vitro effects of POPs on mitochondrial OXPHOS system and suitability of zebrafish as toxicological risk-assessment model to analyze function of mitochondria with toxins.

Unregulated Mitochondrial function exposed in Persistent organic pollutants Mixture

2019 Fall National Meeting

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