The development of new chromatographic materials for high performance liquid chromatography (HPLC) continues to be an important area of research in analytical chemistry. HPLC columns with materials that provide increased stability and new selectivity continue to be in demand. Our research group has developed a new approach to modify silica particles by means of diazotization of <i>p</i>-phenylenediamine, forming a thin phenylene layer containing amino groups. The modified silica particles have been characterized via x-ray photoelectron spectroscopy (XPS) and diffuse reflectance infrared Fourier transform spectroscopy(DRIFT). After surface characterization, the modified particles were packed into HPLC columns. The columns were characterized chromatographically and compared to commercial columns. The columns were also assessed for hydrolytic stability. The columns have shown both hydrophilic and hydrophobic properties that can be exploited for either reversed phase HPLC or hydrophilic interaction liquid chromatography (HILIC). Crosslinking of the phenylene layer further increased hydrolytic stability of the material, showing negligible loss of retention during the time tested. This presentation will provide details of the synthetic approach, characterization, and chromatographic behavior of the newly developed phenylene phase on superficially porous silica particles.

Development of new stationary phases for HPLC

2019 Fall National Meeting

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