The photo physical properties of Cyanine-5 (Cy5) in aqueous and confined reverse-micellar environments were investigated by using steady-state UV-Vis absorption and emission and time-resolved fluorescence spectroscopic techniques. For these studies, the fluorescent dye molecules were encapsulated in both anionic sodium-di-2ethylhexyl sulfosuccinate (AOT) reverse micelles and cationic cetyltrimethyl bromide (CTAB) reverse micelles of various sizes. The photo physical properties of the dyes in water as well as in reverse micelles of various sizes were examined by analyzing various photo physical parameters such as quantum yields, band shifts and fluorescence life time etc. Our observation provides us the future protocols to use reverse micelles as simple model systems, which can mimic the complex biological systems and will help us to understand the dynamic properties of biomolecules.

Photophysics of fluorescent probe molecules in confined environments

2019 Fall National Meeting

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