Background: Protein supplements have become a widespread practice amongst athletes and gym users worldwide. However, the use of protein supplements among female gym users in Riyadh, Saudi Arabia has not yet been investigated. 
Objectives: The aim of this study was to investigate the prevalence of protein supplement usage among female gym users and their attitudes towards protein supplementation in Riyadh. 
Methods: In this cross-sectional study, 502 female gym users aged ≥18 years completed a structured, self-administered online questionnaire on the usage of protein supplementation. Socio-demographic information, anthropometry, health status, exercise frequency, attitudes and knowledge regarding protein supplements were obtained.
Results: The overall prevalence rate of protein supplement use among the study respondents was 36.3%. There were significant associations between the use of protein supplements and educational level (p< 0.05), the duration of being a gym member (p< 0.05) and frequency of gym visits per week (p< 0.05). Whey protein was the predominantly used supplement (57.8%) and the reason attributed to the use of protein supplement was muscle building (53.9%). The main sources of information regarding protein supplement use included the internet (30.2%) followed by gym coaches (25.3%).
Conclusions: Our findings demonstrated that a considerable number of females exercising in gyms use protein supplements and most rely on unreliable sources for decision-making of protein supplements use. Nutritional education and consultations regarding protein supplement use are warranted.

Prevalence and attitudes towards using protein supplements among female gym users: An only survey

The gastrointestinal tract is amongst the most densely populated ecosystems on earth, home to an abundant and diverse collection of microbial species, not just bacteria but also Archaea, viruses, fungi etc. Significant interest in this area has been sparked in recent years by an appreciation of the importance of the gut microbiota in the health of the host, and this has led to the microbiome of humans and animals being investigated in depth. Studies have drawn links between host nutrition, the gut microbiota and metabolic and immune functions, which in consequence affect physiology.



The first investigations of the structure of the microbiota were culture-based, however the introduction of sequencing technologies such as Sanger sequencing and subsequent development of 454 sequencing and metagenomics approaches have become the mainstay of microbiota studies. The creation of reference databases and analysis software has provided invaluable tools for assigning taxonomy to generated data. Great progress in characterising the structure of the microbiome recently has paved the way for ongoing and future studies on the functional interactions between the microbiota and the host. Studies on the function of the microbiota are critical in understanding the role of the microbiota in human homeostasis and disease pathogenesis.



The conference will be presenting the latest advancements in gut microbiome and health research. It will bring together experts who are driving technological advancements and striving to understand how nutrition impacts gut microbiome and the interactions between gut microbiome and health.



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Recordings of the conference will be available until 13 April 2021.