ABSTRACT: Design of new drug material through modification of some well known antibiotics to combat pathogenic bacteria must include a complete understanding of matrix regulation, since the human body consists of majorly three types of matrices; solid, semisolid and liquid. Herein, matrix dependent action of ciprofloxacin based polymeric hydrogel scaffold was explored against a new Vibrio species (V. chemaguriensis Iso1), which is resistant to most of the common antibiotics and possess genes that can be linked to pathogenicity. Ciprofloxacin was attached to the polymeric system consisting of hydrophilic polyethylene glycol methyl ether methacrylate (PEGMA) and zwitterionic sulphabetaine methacrylate (SBMA) with antifouling nature via covalent linkage leading to effective polymer antibiotic conjugates (PACs) with linear and hyperbranched architectures. The hyperbranched PAC was transformed to polymeric gel exhibiting greater antibacterial efficacy in solid matrix than that of liquid one with complete bactericidal effect and rod to spherical switching of bacterial cell followed by chain formation via “dual” contact active and release mechanism through sustain removal of thiol terminated ciprofloxacin fragment along with equilibrium swelling and deswelling process (Figure 1). Lower killing efficacy was displayed in liquid matrix with intact cell morphology due to lack of forced contact between cell wall and gel surface as well as entrapment of released bioactive fragment via additional thick exopolysaccharide (EPS) layer, which throws a great challenge to modern medical sciences.

Matrix assisted regulation of antimicrobial properties: mechanistic elucidation with ciprofloxacin-based polymeric hydrogel against Vibrio sp

ACS Spring Meeting 2021

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.