Metal-organic frameworks (MOFs), as a new kind of functional materials, have received tremendous attention due to their permanent porosity, chemical tunable structures and diverse functionalities. Many pioneering works have been established on the applications of MOFs, including separation, energy conversion, gas storage, chemical sensing, detoxification, catalysis and biomedicine. To date, MOFs have been used as novel materials and matrices for sample preparation in matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Two-dimensional (2-D) MOF nanosheets are emerging types of MOFs that have attracted more considerable attention. They have abundant accessible active sites on the surface and large surface area which are significant different from bulk MOFs. We present a specific view of the applications of MOF nanosheets in MALDI MS. As one of the most important and common posttranslational modifications (PTMs), protein phosphorylation and glycosylation has influences on various complex biological processes, containing cell membrane formation, cell metabolism, immune system and autoimmune diseases. Thus, MOF nanosheets was applied as the powerful materials in the analysis of phosphopeptides and glycopeptides, which played vital roles in the MS analysis for multiple biological processes. According to the experimental results, the 2-D MOF nanosheets material exhibited a good selectivity, ultralow detection limit, and good biocompatibility suggested that the MOF nanosheets significantly improved sample preparation for mass spectrometric analysis in phosphoproteomics and glycoproteomics research.

Enrichment of phosphorylated and glycosylated peptides with metal-organic framework nanosheets for proteomics analysis

ACS Spring Meeting 2021

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