Lipids play an important role in the normal metabolism of living bodies.Metabolic dysregulation of lipids are one of the characteristics of human diseases and even malignant tumors. Multi-dimensional analysis of lipid metabolites in serum can excavate abnormal lipid metabolism that is closely related to the development of disease, help to identify potential biomarkers, and enrich the multiomics information of tumor in vitro diagnosis. However, the high-throughput and high-coverage detection of serum lipids based on major mass spectrometry platforms still faces challenges. To this end, based on the SALDI-MS platform, we have developed a polydopamine modified silicon nanowire (PDA-SiNW) mass spectrometer chip, which can achieve high coverage detection of lipid molecules..The experimental process is shown in Figure 1a. Compared with SiNW without PDA modification, PDA-SiNW significantly improves the signal-to-noise ratio of neutral lipids when detecting serum lipid molecules, and expands the detection range of lipids in serum, especially increases the number of triacylglycerol detections. The detection of about 30 kinds of lipids in serum was carried out. The PDA-SiNW array chip can realize high-throughput mass spectrometry detection of serum lipid metabolites, which helps to efficiently discover more information about lipid metabolites. The technology is expected to play an important role in the early diagnosis of diseases.

Polydopamine-modified silicon nanowire mass spectrometry chip and its application in serum lipid detection

ACS Spring Meeting 2021

The official archive and repository for all ACS Meetings & Expositions abstracts and select posters or presentations.